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Selected Reaction Monitoring Applied to Quantitative Proteomics

  • Reiko Kiyonami
  • Bruno Domon
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 658)

Abstract

Proteomics is gradually shifting from pure qualitative studies (protein identification) to large-scale quantitative experiments, prompted by the growing need to analyze consistently and precisely a large set of proteins in biological samples. The selected reaction monitoring (SRM) technique is increasingly applied to quantitative proteomics because of its selectivity (two levels of mass selection), its sensitivity (non-scanning mode), and its wide dynamic range. This account describes the different steps in the design and the experimental setup of SRM experiments.

Key words

Selective reaction monitoring (SRM) quantitative proteomics triple quadrupole isotopically labeled internal standards 

Notes

Acknowledgments

Dr. Andreas Huhmer and David Fischer are gratefully acknowledged for helpful discussion.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Reiko Kiyonami
    • 1
  • Bruno Domon
    • 2
  1. 1.ThermoFisher ScientificSan JoseUSA
  2. 2.IMSB – ETH ZurichZurichSwitzerland

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