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Bioinformatics for LC-MS/MS-Based Proteomics

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LC-MS/MS in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 658))

Abstract

Mass spectrometry instrumentation has continued to develop rapidly in the last two decades, enabled in part by advances in microelectronic hardware controllers and computerized control and data acquisition systems. The wealth and complexity of data produced by a modern instrument is such that the data can no longer be analyzed manually. Computerized data analysis has become de rigueur and the bioinformatics field has expanded to provide software applications for all aspects of the data analysis needed by LC-MS/MS. The bioinformatics field is evolving rapidly and software applications are continually being improved or replaced for existing applications as well as developed to support new types of experiments and analysis enabled by modern instrumentation. Entire books have been written on MS data analysis in proteomics but this review will be necessarily brief. In this chapter we will review the bioinformatics software applications available for different LC-MS/MS analysis tasks.

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  1. Matrix Science Inc., Boston, MA, USA

    Richard J. Jacob

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  1. Bart’s and the London School of Medicine, Institute of Cancer, Queen Mary, Universtiy of London, Charterhouse Square, London, EC1M 6BQ, United Kingdom

    Pedro R. Cutillas

  2. , EGA Institute for Women's Health, University College London, Gower Street, London, WC1E 6BT, United Kingdom

    John F. Timms

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Jacob, R.J. (2010). Bioinformatics for LC-MS/MS-Based Proteomics. In: Cutillas, P., Timms, J. (eds) LC-MS/MS in Proteomics. Methods in Molecular Biology, vol 658. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-780-8_4

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