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Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics

  • Pedro R. Cutillas
  • John F. Timms
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 658)

Abstract

LC-MS is a powerful technique in biomolecular research. In addition to its uses as a tool for protein and peptide quantization, LC-MS can also be used to quantify the activity of signalling and metabolic pathways in a multiplex and comprehensive manner, i.e. as an ‘activitomic’ tool. Taking cancer research as an illustrative example of application, this review discusses the concepts of biochemical pathway analysis using LC-MS-based proteomic and activitomic techniques.

Key words

Mass spectrometry proteomics metabolomics lipidomics activitomics quantification 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Pedro R. Cutillas
    • 1
  • John F. Timms
    • 2
  1. 1.Analytical Signalling Group, Centre for Cell SignallingInstitute of Cancer, Bart’s and the London School of Medicine, Queen Mary University of LondonLondonUK
  2. 2.Cancer Proteomics LaboratoryEGA Institute for Women’s Health, University College LondonLondonUK

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