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Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries

  • Elisa Petrussa
  • Enrico Braidot
  • Marco Zancani
  • Carlo Peresson
  • Alberto Bertolini
  • Sonia Patui
  • Valentino Casolo
  • Sabina Passamonti
  • Francesco Macrì
  • Angelo Vianello
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 643)

Abstract

Flavonoids are a class of secondary metabolites present in large amounts in grapevine (Vitis vinifera L.), which are involved in several aspects of its physiology (e.g. protection against biotic and abiotic stress). Even if the biosynthetic pathways of flavonoid sub-classes have been largely characterised, the mechanisms of their transport and accumulation to the final target sites are still not completely understood. Unanticipated insights have been obtained by probing plant tissues with pure antibodies targeting bilitranslocase (BTL, TCDB # 2.A.65.1.1), a mammalian transporter involved in the absorption and tissue distribution of dietary flavonoids. The occurrence of a BTL homologue has also been found in grape berries, in both tegumental layers of skin and pulp vascular bundles. In the skin, the expression of this protein starts from véraison (starting of the change in colour and softening of berries) and increases up to a maximum at the harvest stage, matching the same temporal pattern of flavonoid accumulation.

Key words

Bilitranslocase Vitis viniferaberry tissues immunolocalisation microsomes SDS-PAGE Western blotting 

Notes

Acknowledgements

Research on this topic was supported by “Tenuta Villanova”, Vineyard and Winery at Farra d’Isonzo (GO), Italy, by Regione Autonoma Friuli-Venezia Giulia (L.R. 26/2005 art. 17) and by Ministero dell’Istruzione, Università e Ricerca (PRIN project 2004070118).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Elisa Petrussa
    • 1
  • Enrico Braidot
    • 1
  • Marco Zancani
    • 1
  • Carlo Peresson
    • 1
  • Alberto Bertolini
    • 1
  • Sonia Patui
    • 1
  • Valentino Casolo
    • 1
  • Sabina Passamonti
    • 2
  • Francesco Macrì
    • 1
  • Angelo Vianello
    • 1
  1. 1.Department of Plant Biology and Protection, Section of Plant BiologyUdine UniversityUdineItaly
  2. 2.Department of Life SciencesUniversity of TriesteTriesteItaly

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