Abstract
The nuclear factor-kappa B (NF-κB) is a multipotent factor involved in many cellular processes such as inflammation, immune response and embryonic development and it can be activated by a large number of stimuli. Consequently, this transcription factor plays a pivotal role in many natural processes but also in different pathologies. For several years, photodynamic therapy (PDT) has emerged as an attractive alternative approach for the treatment of different affections involving various forms of cancer and an increasing number of reports have highlighted the activation of the NF-κB following PDT treatment. Furthermore, it has been shown that the mechanism of activation of the NF-κB as well as its target genes depends on the nature of the photosensitizers and the cell type used. As this transcription factor is known to be a key regulator of the immune response but also controls cell survival and proliferation, it is important to assess its activation status and its impact on the target genes. In this review, we will present different techniques allowing identification of the activation status of this factor, from the degradation of its inhibitor in the cytoplasm to its ability to induce the expression of a reporter gene under the control of a target promoter. As a working model we will present results obtained from a 5-aminolevulinic acid-PDT treatment on cervix adenocarcinoma cells.
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Luo, J. L., Kamata, H., and Karin, M. (2005) The anti-death machinery in IKK/NF-kappaB signaling. J Clin Immunol, 25(6), 541–550.
Bonizzi, G. and Karin, M. (2004) The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol, 25(6), 280–288.
Ghosh, S. and Hayden, M. S. (2008) New regulators of NF-kappaB in inflammation. Nat Rev Immunol, 8(11), 837–848.
Karin, M. and Greten, F. R. (2005) NF-kappaB linking inflammation and immunity to cancer development and progression. Nat Rev Immunol, 5(10), 749–759.
Ryter, S. W. and Gomer, C. J. (1993) Nuclear factor kappa B binding activity in mouse L1210 cells following Photofrin II-mediated photosensitization. Photochem Photobiol, 58(5), 753–756.
Volanti, C., Gloire, G., Vanderplasschen, A., Jacobs, N., Habraken, Y., and Piette, J. (2004) Downregulation of ICAM-1 and VCAM-1 expression in endothelial cells treated by photodynamic therapy. Oncogene, 23(53), 8649–8658.
Matroule, J. Y., Hellin, A. C., Morliere, P. et al. (1999) Role of nuclear factor-kappa B in colon cancer cell apoptosis mediated by aminopyropheophorbide photosensitization. Photochem Photobiol, 70(4), 540–548.
Granville, D. J., Carthy, C. M., Jiang, H. et al. (2000) Nuclear factor-kappaB activation by the photochemotherapeutic agent verteporfin. Blood, 95(1), 256–262.
Volanti, C., Hendrickx, N., Van Lint, J., Matroule, J. Y., Agostinis, P., and Piette, J. (2005) Distinct transduction mechanisms of cyclooxygenase 2 gene activation in tumour cells after photodynamic therapy. Oncogene, 24(18), 2981–2991.
Matroule, J. Y., Volanti, C., and Piette, J. (2006) NF-kappaB in photodynamic therapy: discrepancies of a master regulator. Photochem Photobiol, 82(5), 1241–1246.
Volanti, C., Matroule, J. Y., and Piette, J. (2002) Involvement of oxidative stress in NF-kappaB activation in endothelial cells treated by photodynamic therapy. Photochem Photobiol, 75(1), 36–45.
Matroule, J. Y., Bonizzi, G., Morliere, P. et al. (1999) Pyropheophorbide-a methyl ester-mediated photosensitization activates transcription factor NF-kappaB through the interleukin-1 receptor-dependent signaling pathway. J Biol Chem, 274(5), 2988–3000.
Acknowledgments
IC is supported by a grant FRIA from the FNRS (Brussels, Belgium), JP is Research Director from the FNRS (Brussels, Belgium) and SB is supported by a grant from the Walloon Region (Belgium).
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Coupienne, I., Piette, J., Bontems, S. (2010). How to Monitor NF-κB Activation After Photodynamic Therapy. In: Gomer, C. (eds) Photodynamic Therapy. Methods in Molecular Biology, vol 635. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-697-9_6
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DOI: https://doi.org/10.1007/978-1-60761-697-9_6
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Publisher Name: Humana Press, Totowa, NJ
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