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Cellular Programming and Reprogramming pp 317–327Cite as

Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 636))

Abstract

Embryonic zebrafish have long been used for lineage-tracing studies. In zebrafish embryos, the cell fate identities can be determined by whole-mount in situ hybridization, or by visualization of live embryos if using fluorescent reporter lines. We use embryonic zebrafish to study the effects of a leukemic oncogene AML1-ETO on modulating hematopoietic cell fate. Induced expression of AML1-ETO is able to efficiently reprogram hematopoietic progenitor cells from erythroid to myeloid cell fate. Using the zebrafish model of AML1-ETO, we performed a chemical screen to identify small molecules that suppress the cell fate switch in the presence of AML1-ETO. The methods discussed herein may be broadly applicable for identifying small molecules that modulate other cell fate decisions.

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References

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Acknowledgments

The authors would like to thank Dr. Randall T. Peterson for his advice and support during the development of this project. J.-R. J. Yeh is supported by a Career Development Award (AG031300) from the National Institute of Aging. This work was supported by RO1 CA118498 and the Claflin Distinguished Scholar Award.

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Authors and Affiliations

  1. Developmental Biology Laboratory, Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA, USA

    Jing-Ruey J. Yeh & Kathleen M. Munson

  2. Department of Medicine, Harvard Medical School, Boston, MA, USA

    Jing-Ruey J. Yeh & Kathleen M. Munson

Authors
  1. Jing-Ruey J. Yeh
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  2. Kathleen M. Munson
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Corresponding author

Correspondence to Jing-Ruey J. Yeh .

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Editors and Affiliations

  1. The Scripps Research Institute, N. Torrey Pines Road 10550, La Jolla, 92037, U.S.A.

    Sheng Ding

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Yeh, JR.J., Munson, K.M. (2010). Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation. In: Ding, S. (eds) Cellular Programming and Reprogramming. Methods in Molecular Biology, vol 636. Humana Press. https://doi.org/10.1007/978-1-60761-691-7_20

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  • DOI: https://doi.org/10.1007/978-1-60761-691-7_20

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-690-0

  • Online ISBN: 978-1-60761-691-7

  • eBook Packages: Springer Protocols

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