Abstract
Cell migration is important in several biological phenomena, such as cancer metastasis. Therefore, the identification of genes involved in cell migration might facilitate the discovery of antimetastatic drugs. However, screening of genes by the current methods can be complicated by factors related to cell stimulation, for example, abolition of contact inhibition and the release inflammatory cytokines from wounded cells during examinations of wound healing in vitro. To overcome these problems and identify genes involved in cell migration, in this chapter we describe the use of transfection microarrays for high-throughput phenotypic screening.
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Acknowledgment
This study was performed as part of “Analytical technology development of dynamic networks inside living cells/Technology development for the real time analysis of the network in the living cells,” funded by the New Energy and Industrial Technology Development Organization (NEDO) of Japan.
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Onuki-Nagasaki, R. et al. (2010). Transfection Microarrays for High-Throughput Phenotypic Screening of Genes Involved in Cell Migration. In: Sioud, M. (eds) RNA Therapeutics. Methods in Molecular Biology, vol 629. Humana Press. https://doi.org/10.1007/978-1-60761-657-3_13
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DOI: https://doi.org/10.1007/978-1-60761-657-3_13
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