Random Mutagenesis Using a Mutator Strain
Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of introducing random point mutations throughout the gene with a fairly high mutation rate. The whole process involves transformation and propagation of a plasmid containing the desired gene into the XL-1 red strain, isolating the mutagenized plasmid library and transforming this library into a desired strain for screening the mutant phenotype.
Key wordsXL1-red™ Random mutation Mutagenized library E. coli Spontaneous mutation DNA repair
- 1.Rupp WD (1996) DNA repair mechanisms. In: Neidhardt FC, Curtiss R III, Ingraham JL, Lin ECC, Low KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella: cellular and molecular biology, 2nd edn. ASM, Washington, DC, pp 2277–2294Google Scholar
- 7.Radman M, Wagner RE, Glickman W, Meselson M (1980) In: Alacevic M (ed) Progress in environmental mutagenesis. Elsevier, Amsterdam, pp 121–130Google Scholar