Random Mutagenesis Using a Mutator Strain

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 634)

Abstract

Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of introducing random point mutations throughout the gene with a fairly high mutation rate. The whole process involves transformation and propagation of a plasmid containing the desired gene into the XL-1 red strain, isolating the mutagenized plasmid library and transforming this library into a desired strain for screening the mutant phenotype.

Key words

XL1-red™ Random mutation Mutagenized library E. coli Spontaneous mutation DNA repair 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory of Transcription BiologyCenter for DNA Fingerprinting and DiagnosticsHyderabadIndia

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