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Detection of Changes in Global Genome Methylation Using the Cytosine-Extension Assay

  • Alex BoykoEmail author
  • Igor Kovalchuk
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)

Abstract

Methylation is a reversible covalent chemical modification of DNA intended to regulate gene expression, genome stability, and chromatin structure. Although there are various methods of methylation analysis, most of them are either laborious or expensive, or both. Here, we describe a quick, inexpensive method for analysis of global genome methylation using a cytosine extension assay. The assay can be used for analysis of the total level of CpG, CNpG, and asymmetrical methylation in a given cell culture or in a plant tissue sample.

Key words

Global genome methylation Cytosine-extension CpG CNpG Asymmetrical methylation 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Biological SciencesUniversity of LethbridgeLethbridgeCanada

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