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Analysis of Bisulfite Sequencing Data from Plant DNA Using CyMATE

  • Andrea M. Foerster
  • Jennifer Hetzl
  • Christoph Müllner
  • Ortrun Mittelsten ScheidEmail author
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)

Abstract

Amplifying and sequencing DNA after bisulfite treatment of genomic DNA reveals the methylation state of cytosine residues at the highest resolution possible. However, a thorough analysis is required for statistical evaluation of methylation at all sites in each genomic region. Several software tools were developed to assist in quantitative evaluation of bisulfite sequencing data from complex methylation patterns occurring in plants. This chapter describes the application of Cytosine Methylation Analysis Tool for Everyone (CyMATE). From aligned sequences, CyMATE quantifies and illustrates general and pattern-specific methylation at CG, CHG, and CHH (H = A, C, or T) sites, both per sequence and per position. CyMATE is also able to perform a quality control of sequences and to detect redundancy among individual clones. The software is able to reveal methylation patterns on complementary strands by handling data from hairpin bisulfite sequencing. The tool is freely available for non-commercial use at http://www.cymate.org.

Key words

DNA methylation 5-methylcytosine (5mC) Bisulfite sequencing Hairpin sequencing Symmetric/asymmetric DNA methylation Methylation context CyMATE 

References

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Andrea M. Foerster
    • 1
  • Jennifer Hetzl
    • 2
  • Christoph Müllner
    • 2
  • Ortrun Mittelsten Scheid
    • 1
    Email author
  1. 1.Gregor Mendel Institute of Molecular Plant BiologyAustrian Academy of SciencesViennaAustria
  2. 2.Institute for Computer Graphics and AlgorithmsVienna University of TechnologyViennaAustria

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