Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein–DNA Binding Analyses in Arabidopsis

  • Stéphane Pien
  • Ueli Grossniklaus
Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)


Epigenetic gene regulation via histone modifications controls different processes ranging from embryonic development, vegetative development, floral induction, floral organ development, to pollen tube growth. The identification of an increasing number of epigenetically regulated processes was greatly advanced by genome-wide histone modification and chromatin-protein interaction surveys. However, genome-wide approaches are too global to access in detail a large number of histone modifications taking place at a single locus. Here we provide a robust Chromatin Immunoprecipitation (ChIP) protocol, allowing in vivo analyses of multiple chromatin modifications and binding of histone modifiers in different plant organs and tissues. This method is quantitative and provides a way to study the dynamic state of chromatin during plant development and also in response to different environmental stimuli.

Key words

Arabidopsis Chromatin Histone modification Epigenetic ChIP Protein-DNA interaction 


  1. 1.
    Pien S, Grossniklaus U (2007) Polycomb group and trithorax group proteins in Arabidopsis. Biochem Biophys Acta 1769:375–382PubMedGoogle Scholar
  2. 2.
    Orlando V, Paro R (1993) Mapping Polycomb-repressed domains in the BX-C using in vivo formaldehyde crosslinked chromatin. Cell 75:1187–1198CrossRefPubMedGoogle Scholar
  3. 3.
    Boyko A, Kovalchuk I (2008) Epigenetic control of plant stress response. Environ Mol Mutagen 49:61–72CrossRefPubMedGoogle Scholar
  4. 4.
    Madlung A, Comai L (2004) The effect of stress on genome regulation and structure. Ann Bot (Lond) 94:481–495CrossRefGoogle Scholar
  5. 5.
    Pien S, Fleury D, Mylne JS, Crevillen P, Inzé D, Avramova Z et al (2008) ARABIDOPSIS TRITHO­RAX1 dynamically regulates FLOWERING LOCUS C activation via histone 3 lysine 4 trimethylation. Plant Cell 20:580–588CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Stéphane Pien
    • 1
  • Ueli Grossniklaus
    • 1
  1. 1.Institute of Plant Biology & Zürich-Basel Plant Science CenterUniversity of ZürichZürichSwitzerland

Personalised recommendations