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Metaanalysis of ChIP-chip Data

  • Julia Engelhorn
  • Franziska Turck
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 631)

Abstract

Genome-wide analysis of histone modifications via ChIP-chip (chromatin immunoprecipitation followed by whole genome tiling array hybridization) may generate lists of up to several thousand potential target genes. In the case of the model organism Arabidopsis thaliana, several databases are available to alleviate further characterization and classification of genomic data sets. The term metaanalysis has been coined for this type of multidatabase comparison. In this chapter, we describe open source software and web tools that perform transcriptional and functional analysis of target genes. Sources of transcription data and clustering tools to subdivide genes according to their expression pattern are described. The user is guided through all necessary steps, including data download and formatting. In addition, the Gene Ontology (GO) vocabulary and methods to uncover over- or underrepresented functions among target genes are introduced. Genomic targets of the histone H3K27me3 modification are presented as a case study to demonstrate that metaanalysis can uncover novel functions that were hidden in genomic data sets.

Key words

Metaanalysis AtGenExpress Hierarchical clustering K-means clustering Gene ontology Functional enrichment analysis 

Notes

Acknowledgments

We thank Drs. Seth Davis and Anika Jöcker for critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Julia Engelhorn
    • 1
  • Franziska Turck
    • 1
  1. 1.Max Planck Institute for Plant Breeding ResearchKölnGermany

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