Abstract
Carbon nanotubes (CNTs) have a great potential for applications in medicine. However, their biocompatibility and toxicity cause a great concern. Due to the large surface area of CNTs, chemical modification can dramatically alter their physiochemical properties and hence biological activity. Using a combinatorial chemistry approach, we report the synthesis of an 80-member surface-modified nanotube library. Based upon screening of this library with respect to protein-binding capacity, cytotoxicity, and immune response, we were able to select highly biocompatible nanotubes with reduced protein-binding cytotoxicity and immune response.
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References
Mattson MP, Haddon RC, Rao AM (2000) Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 14:175-182
Liu Z, Cai WB, He LN, Nakayama N, Chen K, Sun XM et al (2007) In vivo biodistribution and highly efficient tumour targeting of carbon nanotubes in mice. Nat Nanotechnol 2:47-52
Singh R, Pantarotto D, Lacerda L, Pastorin G, Klumpp C, Prato M et al (2006) Tissue biodistribution and blood clearance rates of intravenously administered carbon nanotube radiotracers. Proc Natl Acad Sci U S A 103:3357-3362
Wang HF, Wang J, Deng XY, Sun HF, Shi ZJ, Gu ZN et al (2004) Biodistribution of carbon single-wall carbon nanotubes in mice. J Nanosci Nanotechnol 4:1019-1024
Cherukuri P, Bachilo SM, Litovsky SH, Weisman RB (2004) Near-infrared fluorescence microscopy of single-walled carbon nanotubes in phagocytic cells. J Am Chem Soc 126:15638-15639
Kam NWS, Dai HJ (2005) Carbon nanotubes as intracellular protein transporters: generality and biological functionality. J Am Chem Soc 127:6021-6026
Karajanagi SS, Vertegel AA, Kane RS, Dordick JS (2004) Structure and function of enzymes adsorbed onto single-walled carbon nanotubes. Langmuir 20:11594-11599
Zheng M, Jagota A, Semke ED, Diner BA, McLean RS, Lustig SR et al (2003) DNA-assisted dispersion and separation of carbon nanotubes. Nat Mater 2:338-342
Nahar M, Dutta T, Murugesan S, Asthana A, Mishra D, Rajkumar V et al (2006) Functional polymeric nanoparticles: an efficient and promising tool for active delivery of bioactives. Crit Rev Ther Drug Carrier Syst 23:259-318
Colvin VL, Kulinowski KM (2007) Nanoparticles as catalysts for protein fibrillation. Proc Natl Acad Sci U S A 104:8679-8680
Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H et al (2007) Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc Natl Acad Sci U S A 104:2050-2055
Vertegel AA, Siegel RW, Dordick JS (2004) Silica nanoparticle size influences the structure and enzymatic activity of adsorbed lysozyme. Langmuir 20:6800-6807
Lynch I, Dawson KA, Linse S (2006) Detecting cryptic epitopes created by nanoparticles. Sci STKE 2006:p14
Owens DE, Peppas NA (2006) Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles. Int J Pharm 307:93-102
Zhou H, Mu Q, Gao N, Liu A, Xing Y, Gao S et al (2008) A nano-combinatorial library strategy for the discovery of nanotubes with reduced protein-binding, cytotoxicity, and immune response. Nano Lett 8:859-865
Worle-Knirsch JM, Pulskamp K, Krug HF (2006) Oops they did it again! Carbon nanotubes hoax scientists in viability assays. Nano Lett 6:1261-1268
Pulskamp K, Diabate S, Krug HF (2007) Carbon nanotubes show no sign of acute toxicity but induce intracellular reactive oxygen species in dependence on contaminants. Toxicol Lett 168:58-74
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15 N] nitrate in biological fluids. Anal Biochem 126:131-138
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Zhang, Q., Zhou, H., Yan, B. (2010). Reducing Nanotube Cytotoxicity Using a Nano-Combinatorial Library Approach. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_9
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DOI: https://doi.org/10.1007/978-1-60761-579-8_9
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