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Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 625)

Abstract

We describe in detail the different steps involved in the construction of a carbon nanotube field-effect transistor (CNTFET) based on a network of single-walled carbon nanotubes (SWCNTs), which can selectively detect human immunoglobulin G (HIgG). HIgG antibodies, which are strongly adsorbed onto the walls of the SWCNTs, are the basic elements of the recognition layer. The nonspecific binding of proteins or other interferences are avoided by covering the nonadsorbed areas of the SWCNTs with Tween 20. The CNTFET is a reagentless device that does not need labels to detect HIgG.

Key words

Carbon nanotubes Field-effect transistor CNTFET HIgG 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Analytical and Organic ChemistryUniversitat Rovira i VirgiliTarragonaSpain

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