Abstract
High-density carbon nanotube (CNT)-coated surfaces are highly neuro-adhesive. When shaped into regular arrays of isolated islands on a non-adhesive support substrate (such as a clean glass), CNTs can function as effective encoring sites for neurons and glia cells for in-vitro applications. Primarily, patterned CNT islands provide a means to form complex, engineered, interconnected neuronal networks with pre-designed geometry via utilizing the self-assembly process of neurons. Depositing these CNT islands onto multielectrode array chip can facilitate both cell anchoring but also electrical interfacing between the electrodes and the neurons.
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References
Fan YW, Cui FZ, Hou SP, Xu QY, Chen LN, Lee IS (2002) Culture of neural cells on silicon wafers with nano-scale surface topography. J Neurosci Methods 120:17-23
Goldner JS, Bruder JM, Li G, Gazzola D, Hoffman-Kim D (2006) Neurite bridging across micropatterned grooves. Biomaterials 27:460-472
Johansson F, Carlberg P, Danielsen N, Montelius L, Kanje M (2006) Axonal outgrowth on nano-imprinted patterns. Biomaterials 27:1251-1258
Curtis AS (2004) Small is beautiful but smaller is the aim: review of a life of research. Eur Cell Mater 8:27-36
Zhang X, Prasad S, Niyogi S, Morgan A, Ozkan M, Ozkan CS (2005) Guided neurite growth on patterned carbon nanotubes. Sensor Actuators B 106:843-850
Harrison BS, Atala A (2007) Carbon nanotube applications for tissue engineering. Biomaterials 28:344-353
Malarkey EB, Parpura V (2007) Applications of carbon nanotubes in neurobiology. Neurodegener Dis 4:292-299
Mattson MP, Haddon RC, Rao AM (2000) Molecular functionalization of carbon nanotubes and use as substrates for neuronal growth. J Mol Neurosci 14:175-182
Hu H, Ni YC, Mandal SK, Montana V, Zhao N, Haddon RC et al (2005) Polyethyleneimine functionalized single-walled carbon nanotubes as a substrate for neuronal growth. J Phys Chem B 109:4285-4289
Hu H, Ni YC, Montana V, Haddon RC, Parpura V (2004) Chemically functionalized carbon nanotubes as substrates for neuronal growth. Nano Lett 4:507-511
Gabay T, Ben-David M, Kalifa I, Sorkin R, Abrams ZR, Ben-Jacob E et al (2007) Electro-chemical and biological properties of carbon nanotube based multi-electrode arrays. Nanotechnology 18:035201-035206
Gabay T, Jakobs E, Ben-Jacob E, Hanein Y (2005) Engineered self-organization of neural networks using carbon nanotube clusters. Physica A 350:611-621
Wang K, Fishman HA, Dai HJ, Harris JS (2006) Neural stimulation with a carbon nanotube microelectrode array. Nano Lett 6:2043-2048
Mazzatenta A, Giugliano M, Campidelli S, Gambazzi L, Businaro L, Markram H et al (2007) Interfacing neurons with carbon nanotubes. Electrical signal transfer and synaptic stimulation in cultured brain circuits. J Neurosci 27:6931-6936
Lovat V, Pantarotto D, Lagostena L, Cacciari B, Grandolfo M, Righi M et al (2005) Carbon nanotube substrates boost neuronal electrical signaling. Nano Lett 5:1107-1110
Nguyen-Vu TDB, Chen H, Cassell AM, Andrews R, Meyyappan M, Li J (2006) Vertically aligned carbon nanofiber arrays: an advance toward electrical-neural interfaces. Small 2:89-94
Sorkin R, Gabay T, Blinder P, Baranes D, Ben-Jacob E, Hanein Y (2006) Compact self-wiring in cultured neural networks. J Neural Eng 3:95-101
Gabay T, Ben-David M, Kalifa I, Sorkin R, Abrams ZR, Ben-Jacob E, Hanein Y (2007) Electro-chemical and biological properties of carbon nanotube based multi-electrode arrays. Nanotechnology 18:035201-035206
Ben-Jacob E, Hanein Y (2008) Carbon nanotube micro-electrodes for neuronal interfacing. J Mater Chem 18:5181
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The authors thank Inna Brains for technical assistance.
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David-Pur, M., Shein, M., Hanein, Y. (2010). Carbon Nanotube-Based Neurochips. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_14
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DOI: https://doi.org/10.1007/978-1-60761-579-8_14
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