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Carbon Nanotubes pp 153–168Cite as

Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied by Capillary Electrophoresis–Laser–Induced Fluorescence

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 625))

Abstract

Fluorescently labeled carbon nanotube probes (CNTP) are prepared by derivatizing oxidized (o)-MWNTs with a fluorescein dye. Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) detection is used to separate and detect CNTP in multidrug-resistant cells (K562A) and the parent cells (K562S). CE-LIF and flow cytometry investigation reveal that the CNTP can traverse the membranes in both cell lines without being pumped out by P-glycoprotein. The CE-LIF method is also useful for quantitative analysis of CNT in single cells, enabling drug delivery and multidrug resistance (MDR) studies. Moreover, toward quantifying the intracellular uptake of oxidized (o)-SWNTs with anchored Rhodamine123 (Rho123), fluorescence-quenching of Rho123 is measured by micellar electrokinetic chromatography coupled with LIF detection. Enhanced uptake of Rho123 in multidrug-resistant leukemia cells can be achieved with the aid of the o-SWNTs carriers. Besides being able to overcome MDR, o-SWNTs are shown to be excellent intracellular carriers possessing large adsorption capacity and prolonged release ability. Finally, it is demonstrated that o-SWNTs are safe for biological applications at concentrations of up to 40 µg/mL.

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Acknowledgments

Financial support from the National Natural Sciences Foundation of China (No. 20520120220), the China State Key Basic Research Program Grants (2003CB716002), and the Knowledge Inno­vation Program of DICP to H.Z. are gratefully acknowledged.

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Authors and Affiliations

  1. National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, P.R., China

    Ruibin Li, Hanfa Zou, Hua Xiao & Renan Wu

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  1. Ruibin Li
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  2. Hanfa Zou
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  3. Hua Xiao
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  4. Renan Wu
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Corresponding author

Correspondence to Ruibin Li .

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Editors and Affiliations

  1. MPI für Festkörperforschung, Heisenbergstr. 1, Stuttgart, 70569, Germany

    Kannan Balasubramanian

  2. MPI für Festkörperforschung, Heisenbergstr. 1, Stuttgart, 70569, Germany

    Marko Burghard

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Li, R., Zou, H., Xiao, H., Wu, R. (2010). Carbon Nanotubes as Intracellular Carriers for Multidrug Resistant Cells Studied by Capillary Electrophoresis–Laser–Induced Fluorescence. In: Balasubramanian, K., Burghard, M. (eds) Carbon Nanotubes. Methods in Molecular Biology, vol 625. Humana Press. https://doi.org/10.1007/978-1-60761-579-8_13

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  • DOI: https://doi.org/10.1007/978-1-60761-579-8_13

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-577-4

  • Online ISBN: 978-1-60761-579-8

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