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Integrase Defective, Nonintegrating Lentiviral Vectors

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Lentivirus Gene Engineering Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 614))

Abstract

Lentiviral vectors are a powerful tool for gene transfer into target cells in vitro and in vivo. However, there are concerns about safety with regard to their use in gene transfer protocols because of insertional mutagenesis following viral infection. Once in the target cells, and in addition to the integrated proviral DNA, lentiviral vectors produce episomal forms of DNA (E-DNA), which are transcriptionally active. Therefore, one strategy to improve safety would envision the block integration of the lentiviral vector while allowing production of E-DNA. Such nonintegrating lentiviral vectors can be produced by introducing mutations in the Integrase (IN) protein of the parental packaging vector. These vectors are fundamentally different from the parental IN competent counterpart, thus opening new avenues for this class of lentiviral vectors as a new gene delivery system for gene therapy strategies, vaccination protocols and as a tool for anti-Integrase drug discovery.

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Acknowledgments

The authors would like to thank Patrizia Cocco and Ferdinando Costa (National AIDS Center, Istituto Superiore di Sanità, Rome, Italy) for technical support.

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Authors and Affiliations

  1. Department of Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy

    Zuleika Michelini & Andrea Cara

  2. Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy

    Donatella Negri

Authors
  1. Zuleika Michelini
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  2. Donatella Negri
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  3. Andrea Cara
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Corresponding author

Correspondence to Andrea Cara .

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Editors and Affiliations

  1. Div. Patogenesi dei Retrovirus, Centro Nazionale AIDS, Viale Regina Elena 299, Roma, 00161, Italy

    Maurizio Federico

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Michelini, Z., Negri, D., Cara, A. (2010). Integrase Defective, Nonintegrating Lentiviral Vectors. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology, vol 614. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-533-0_6

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  • DOI: https://doi.org/10.1007/978-1-60761-533-0_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-532-3

  • Online ISBN: 978-1-60761-533-0

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