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Calcium Measurement Methods pp 57–80Cite as

Ca2+ Imaging: Principles of Analysis and Enhancement

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Part of the book series: Neuromethods ((NM,volume 43))

Abstract

In this chapter, we review the theoretical and experimental foundations underling a quantitative approach to Ca2+ imaging, discuss equilibrium conditions and their violations and present a computational framework that can be used to estimate the spatial and temporal dynamics of Ca2+ signals based of fluorescence measurements with Ca2+ indicators.

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Notes

  1. 1.

    Note that, because of (3.3), the c T in (3.4) is the same as in (3.8)–(3.10).

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Acknowledgments

This work has been supported by grants to FM from Fondazione Cariparo (Progetti di Eccellenza 2006), MIUR PRIN Grant n. 2007BZ4RX3_003 and the European commission FP6 Integrated Project EuroHear (LSHGCT20054512063) under the Sixth Research Frame Program of The European Union. We thank the Editor of this book, Alexej Verkhratsky, for discussions and constructive criticism.

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Authors and Affiliations

  1. Department of Physics, University of Padua, Padua, Italy

    Fabio Mammano & Mario Bortolozzi

  2. Foundation for Advanced Biomedical Research, Venetian Institute of Molecular Medicine, Padua, Italy

    Fabio Mammano & Mario Bortolozzi

Authors
  1. Fabio Mammano
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  2. Mario Bortolozzi
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Corresponding author

Correspondence to Fabio Mammano .

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Editors and Affiliations

  1. School of Biological Sciences, University of Manchester, Oxford Road, Manchester, M13 9PT, United Kingdom

    Alexei Verkhratsky

  2. School of Biomedical Science, University of Liverpool, Crown Street, Liverpool, L69 3BX, United Kingdom

    Ole H. Petersen

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Mammano, F., Bortolozzi, M. (2010). Ca2+ Imaging: Principles of Analysis and Enhancement. In: Verkhratsky, A., Petersen, O. (eds) Calcium Measurement Methods. Neuromethods, vol 43. Humana Press. https://doi.org/10.1007/978-1-60761-476-0_3

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  • DOI: https://doi.org/10.1007/978-1-60761-476-0_3

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-475-3

  • Online ISBN: 978-1-60761-476-0

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