Abstract
Although reverse-phase liquid chromatography (RP-LC) is a common technique for peptide separation in shotgun proteomics and glycoproteomics, it often provides unsatisfactory results for the analysis of glycopeptides and glycans. This bias against glycopeptides makes it difficult to study glycoproteins. By coupling mass spectrometry (MS) with a combination of RP-LC and normal-phase (NP)-LC as an integrated front-end separation system, we demonstrate that effective identification and characterization of both peptides and glycopeptides mixtures, and their constituent glycan structures, can be achieved from a single sample injection event.
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Acknowledgments
This study was supported by the Hong Kong Research Grants Council (Project No. HKU7640/07 M), Hong Kong Special Administrative Region, China.
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Siu, S., Lam, M.P., Lau, E., Yeung, W.S., Cox, D.M., Chu, I.K. (2010). N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry. In: Li, J. (eds) Functional Glycomics. Methods in Molecular Biology, vol 600. Humana Press. https://doi.org/10.1007/978-1-60761-454-8_9
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DOI: https://doi.org/10.1007/978-1-60761-454-8_9
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