Liposomes pp 531-542 | Cite as

Use of Liposomes to Study Vesicular Transport

  • Kohji TakeiEmail author
  • Hiroshi Yamada
  • Tadashi Abe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)


Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.

Key words

Vesicular transport Membrane traffic Endocytosis Phagocytosis 



The work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of NeuroscienceOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayamaJapan

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