Use of Liposomes to Study Vesicular Transport
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
Key wordsVesicular transport Membrane traffic Endocytosis Phagocytosis
The work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan.
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