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Liposomes pp 509-518 | Cite as

Liposome Biodistribution via Europium Complexes

  • Nathalie MignetEmail author
  • Daniel Scherman
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

The drug-delivery field needs tools to follow vector biodistribution. Radioactive tracers and conventional fluorophores are widely used. We propose here to use europium complexes. Use of pulsed light source time-resolved fluorimetry takes into account the fluorescence decay time of the lanthanide chelates to gain sensitivity in biological media. The method was developed to follow liposome biodistribution. Octadecyl-DTPA.Eu compound has been prepared and incorporated into liposomes without alteration of its fluorescence signal. The method has been validated by comparison with fluorophore-labelled liposomes. The way to proceed to use this method for liposomes or other vectors is detailed.

Key words:

Liposomes Europium Time-resolved fluorimetry Fluorescence Pharmacokinetics 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Unité de Pharmacologie Chimique et Génétique; CNRS, UMR 8151ParisFrance
  2. 2.Inserm, U 640ParisFrance
  3. 3.Faculté des Sciences Pharmaceutiques et BiologiquesUniversité Paris DescartesParisFrance
  4. 4.ENSCPParisFrance

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