Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery
The biological activity of cationic liposome/DNA complexes (“lipoplexes”) is strongly dependent on their ability to protect DNA and to interact with cells, including binding to the cell surface, internalization via endocytosis and cytoplasmic delivery of the DNA. In this chapter, we describe a number of methods and procedures to study these processes, based on the use of fluorescent probes.
Key wordsCationic liposomes Lipoplexes Gene delivery DNA protection Binding and uptake of lipoplexes Intracellular distribution of lipoplexes
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