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Liposomes pp 425-437 | Cite as

Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery

  • Henrique Faneca
  • Nejat Düzgüneş
  • Maria C. Pedroso de Lima
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

The biological activity of cationic liposome/DNA complexes (“lipoplexes”) is strongly dependent on their ability to protect DNA and to interact with cells, including binding to the cell surface, internalization via endocytosis and cytoplasmic delivery of the DNA. In this chapter, we describe a number of methods and procedures to study these processes, based on the use of fluorescent probes.

Key words

Cationic liposomes Lipoplexes Gene delivery DNA protection Binding and uptake of lipoplexes Intracellular distribution of lipoplexes 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Henrique Faneca
    • 1
  • Nejat Düzgüneş
    • 2
  • Maria C. Pedroso de Lima
    • 3
  1. 1.Faculty of Science and Technology, Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbraPortugal
  2. 2.Department of Microbiology, Arthur A. Dugoni School of DentistryUniversity of the PacificSan FranciscoUSA
  3. 3.Department of Biochemistry, Faculty of Science and Technology, Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbraPortugal

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