Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes
Lipoplex preparations are heterogeneous mixtures of lipoplex particles of different structures. As these structures determine the efficiency of the delivery of genetic material, it is important to characterize the distribution of particles of different types in lipoplex preparations with good statistics. We describe the application of flow fluorometry which allows producing such distributions (in terms of lipoplex particle size and composition) within minutes using basic flow cytometer (Anal Biochem 341:230-240, 2005).
Key wordsFlow cytometry Lipoplex Cationic lipid Heterogeneity Single particle detection Size-stoichiometry distribution
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