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Liposomes pp 385-391 | Cite as

Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes

  • Edwin Pozharski
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

Lipoplex preparations are heterogeneous mixtures of lipoplex particles of different structures. As these structures determine the efficiency of the delivery of genetic material, it is important to characterize the distribution of particles of different types in lipoplex preparations with good statistics. We describe the application of flow fluorometry which allows producing such distributions (in terms of lipoplex particle size and composition) within minutes using basic flow cytometer (Anal Biochem 341:230-240, 2005).

Key words

Flow cytometry Lipoplex Cationic lipid Heterogeneity Single particle detection Size-stoichiometry distribution 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Edwin Pozharski
    • 1
  1. 1.Department of Pharmaceutical SciencesUniversity of MarylandBaltimoreUSA

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