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Liposomes pp 363-368 | Cite as

Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID

  • Sophia HatziantoniouEmail author
  • Costas Demetzos
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

Liposomes are composed of different kind of lipids or lipophilic substances and are used as carriers of bioactive molecules. The characterization of the prepared liposomes consists of the calculation of the drug to lipid molar ratio by measuring the lipids and the encapsulated molecule.

The present work describes an analytical methodology on simultaneous determination of all the lipid ingredients of the liposome formulation, using Thin Layer Chromatography coupled with a Flame Ionization Detector (TLC/FID), using the least possible sample quantity. The method consists of a chromatographic separation of the liposomal ingredients on silica gel scintillated on quartz rods and subsequent detection of the ingredients by scanning the rods by a hydrogen flame. The produced ions are detected by a Flame Ionization Detector and the signal is converted to a chromatogram.

This method may be applied on every step of the liposome preparation for examining the quality of the raw materials, tracking possible errors of the preparation procedure and finally analyzing the content of the final liposomal composition.

Key words

Liposome Lipid analysis Drug/lipid ratio Bioactive molecule HPTLC/FID 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Pharmaceutical Technology, School of PharmacyUniversity of AthensAthensGreece

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