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Liposomes pp 351-361 | Cite as

Atomic Force Microscopy for the Characterization of Proteoliposomes

  • Johannes Sitterberg
  • Maria Manuela Gaspar
  • Carsten Ehrhardt
  • Udo Bakowsky
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

Atomic Force Microscopy (AFM) is a useful tool for the visualization of soft biological samples in a nanoscale resolution. In the study presented here, the surface morphology ofP-selectin and Transferrin modified proteoliposomes were investigated in air and under water. The proteins were visualized without pre-functionalization or staining.

Key words

AFM Proteoliposomes P-Selectin Transferrin Surface Modification 

Notes

Acknowledgments

This work was supported in parts by DFG Forschergruppe 627 Nanohale and JPK Instruments Berlin (Germany) (UB and JS). This work was supported in part by grants from Enterprise Ireland under the National Development Plan co-funded by EU Structural Funds and Science Foundation Ireland (CE and MMG).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Johannes Sitterberg
    • 1
  • Maria Manuela Gaspar
    • 2
  • Carsten Ehrhardt
    • 3
  • Udo Bakowsky
    • 4
  1. 1.Department of Pharmaceutical Technology and BiopharmacyPhilipps-Universität MarburgMarburgGermany
  2. 2.Unidade Novas Formas de Agentes Bioactivos, iMed, Faculdade de FarmáciaUniversidade de LisboaLisboaPortugal
  3. 3.School of Pharmacy and Pharmaceutical SciencesUniversity of Dublin, Trinity College DublinDublinIreland
  4. 4.Department of Pharmaceutical Technology and BiopharmacyPhilipps-Universität MarburgMarburgGermany

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