Liposomes pp 199-207 | Cite as

Assessment of Liposome–Cell Interactions

  • Jan A. A. M. KampsEmail author
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)


Liposome-cell interactions have been assessed for over 30 years now by an enormous variety of approaches and methods. In-depth knowledge of liposome-cell interaction is still very relevant since new concepts and applications applying liposomes are being developed every day. This chapter does not aim to give a complete overview on methods to assess liposome-cell interactions but merely gives a handle to approach the assessment of liposome-cell interaction by describing some well-established methods that also allow for modification to adapt them for your specific research questions.

Key words

Endocytosis Fluorescence microscopy Radioactive liposome markers Fluorescent liposome markers Cell binding Liposome uptake Cellular processing of liposomes 


  1. 1.
    Pagano RE, Weinstein JN (1978) Interactions of liposomes with mammalian cells. Annu Rev Biophys Bioeng 7:435-468CrossRefPubMedGoogle Scholar
  2. 2.
    Düzgünes N, Nir S (1999) Mechanisms and kinetics of liposome-cell interactions. Adv Drug Deliv Rev 40(1-2):3-18CrossRefGoogle Scholar
  3. 3.
    Kamps JAAM, Scherphof GL (2003) Liposomes in biological systems. In: Torchilin VP, Weissig V (eds) Liposomes, a practical approach, 2nd edn. Oxford University Press, Oxford, pp 267-288Google Scholar
  4. 4.
    Torchilin VP (2005) Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov 4(2):145-160CrossRefPubMedGoogle Scholar
  5. 5.
    Yan X, Poelstra K, Scherphof GL, Kamps JA (2004) Role for scavenger receptor B-I in selective transfer of rhodamine-PE from liposomes to cells. Biochem Biophys Res Commun 325(3):908-914CrossRefPubMedGoogle Scholar
  6. 6.
    Kamps JA, Morselt HW, Scherphof GL (1999) Uptake of liposomes containing phosphatidylserine by liver cells in vivo and by sinusoidal liver cells in primary culture: in vivo-in vitro differences. Biochem Biophys Res Commun 256(1):57-62CrossRefPubMedGoogle Scholar
  7. 7.
    Böttcher CJF, Van Gent CM, Pries C (1961) A rapid and sensitive sub-micro phosphorus determination. Anal Chim Acta 24:203-204CrossRefGoogle Scholar
  8. 8.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275PubMedGoogle Scholar
  9. 9.
    Derksen JT, Morselt HW, Scherphof GL (1988) Uptake and processing of immunoglobulin-coated liposomes by subpopulations of rat liver macrophages. Biochim Biophys Acta 971(2):127-136PubMedGoogle Scholar
  10. 10.
    Kamps JA, Morselt HW, Swart PJ, Meijer DK, Scherphof GL (1997) Massive targeting of liposomes, surface-modified with anionized albumins, to hepatic endothelial cells. Proc Natl Acad Sci U S A 94(21):11681-11685CrossRefPubMedGoogle Scholar
  11. 11.
    Koning GA, Morselt HW, Velinova MJ et al (1999) Selective transfer of a lipophilic prodrug of 5-fluorodeoxyuridine from immunoliposomes to colon cancer cells. Biochim Biophys Acta 1420(1-2):153-167CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory for Endothelial Biomedicine & Vascular Drug Targeting Research, Medical Biology Section, Department Pathology & Medical BiologyUniversity Medical Center GroningenGroningenThe Netherlands

Personalised recommendations