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Liposomes pp 127-145 | Cite as

Biosynthesis of Proteins Inside Liposomes

  • Pasquale Stano
  • Yutetsu Kuruma
  • Tereza Pereira de Souza
  • Pier Luigi LuisiEmail author
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

Protein expression is the most complex metabolic reaction that has been encapsulated in liposomes, mainly as an intermediate step toward the synthesis of minimal semisynthetic cells. Although there are different experimental approaches to achieving the synthesis of proteins inside liposomes and it is therefore not possible to give a standard recipe, all methods follow a general strategy, which is briefly discussed. On this basis, we provide general indications for designing and realizing protein-expressing liposomes. Our approach for the green fluorescent protein expression inside 200-nm extruded vesicles is described in detail.

Key words

Cell-free protein expression PURESYSTEM Enhanced green fluorescent protein (EGFP) Minimal cell Synthetic biology 

Notes

Acknowledgements

This work was carried out within the SYNTHCELLS project (Approaches to the Bioengineering of Synthetic Minimal Cells, EU Grant #FP6-043359) and further supported by the Human Frontiers Science Program.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Pasquale Stano
    • 1
  • Yutetsu Kuruma
    • 2
  • Tereza Pereira de Souza
    • 1
  • Pier Luigi Luisi
    • 1
    Email author
  1. 1.Biology DepartmentUniversity of RomaTreRomeItaly
  2. 2.“Enrico Fermi” Study and Research CenterRomeItaly

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