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Liposomes pp 115-126 | Cite as

Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles

  • Martin StöcklEmail author
  • Jörg Nikolaus
  • Andreas Herrmann
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

Recent studies suggest that phospholipids in the plasma membrane of mammalian cells are not homogenously distributed but may form domains either by lipid-lipid interactions or/and as consequence of lipid-protein interactions. Such lipid compartments may act as protein recruiting platforms which, for example, are essential components of cell signaling pathways. Model membrane systems with a defined lipid composition are ideally suited to study domain-specific interactions of peripheral and integral membrane proteins. Giant unilamellar vesicles (GUVs) offer the opportunity to directly visualize in parallel, both the lateral lipid domains and the interaction sites of proteins using fluorescence microscopy. The application of GUVs is exemplarly illustrated for studying domain-specific interactions of the protein α-synuclein and the domain-specific distribution of synthetic transmembrane peptides.

Key words

Giant unilamellar vesicles (GUVs) Fluorescence microscopy Lipid domain Raft α-Synuclein Transmembrane domain 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Martin Stöckl
    • 1
    Email author
  • Jörg Nikolaus
    • 1
  • Andreas Herrmann
    • 1
  1. 1.Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie/BiophysikHumboldt-Universität zu BerlinBerlinGermany

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