Advertisement

Liposomes pp 1-10 | Cite as

Utilization of Liposomes for Studying Drug Transfer and Uptake

  • Alfred FahrEmail author
  • Xiangli Liu
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 606)

Abstract

On entry into the body of the patient, drugs have to overcome many barriers in order to reach the target. The knowledge of the ability of drugs to cross these barriers, which mostly consist of lipid membranes, is of utmost interest in pharmacy.

High values of lipophilicity of a drug might be a good pre-requisite for crossing these barriers. It also led liposomologists to think that highly lipophilic drugs may “stick” in the lipophilic interior of liposomal phospholipid membranes and therefore these liposomes may act as a retard formulation of the lipophilic drug.

The presented method here estimates the transfer time of lipophilic drugs between liposomal lipid bilayers. This may help to judge the presumed retardation function of a specific liposomal delivery system for a chosen lipophilic drug.

Key words

Membrane transfer Liposome drug delivery system Lipophilic drug Retardation Mini column method 

Notes

Acknowledgment

We thank Rene Schaufelberger (Novartis Pharma Inc., Basel) for excellent technical assistance in setting up these procedures.

References

  1. 1.
    Papahadjopoulos D, Bangham AD (1996) Biophysical properties of phospholipids. II. Permeability of phosphatidyl liquid crystal to univalent ions. Biochim Biophys Acta 126:185-188Google Scholar
  2. 2.
    Flaten GE et al (2006) Drug permeability across a phospholipid vesicle-based barrier 2. Characterization of barrier structure, storage stability and stability towards pH changes. Eur J Pharm Sci 28(4):336-43CrossRefPubMedGoogle Scholar
  3. 3.
    Shabbits JA, Chiu GN, Mayer LD (2002) Development of an in vitro drug release assay that accurately predicts in vivo drug retention for liposome-based delivery systems. J Control Release 84(3):161-70CrossRefPubMedGoogle Scholar
  4. 4.
    Fahr A et al (2005) Transfer of lipophilic drugs between liposomal membranes and biological interfaces: consequences for drug delivery. Eur J Pharm Sci 26:251-265CrossRefPubMedGoogle Scholar
  5. 5.
    Joguparthi V, Xiang TX, Anderson BD (2008) Liposome transport of hydrophobic drugs: gel phase lipid bilayer permeability and partitioning of the lactone form of a hydrophobic camptothecin, DB-67. J Pharm Sci 97(1):400-20CrossRefPubMedGoogle Scholar
  6. 6.
    Bienveue A et al (1985) Kinetics of phospholipid transfer between liposomes (neutral or negatively charged) and high-density lipoproteins: a spin-label study of early events. Biochim Biophys Acta 835:557-566Google Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of PharmaceuticsFriedrich-Schiller-UniversityJenaGermany

Personalised recommendations