Abstract
Ecophysiological analysis and functions of single cells in complex microbial systems can be examined by simple combinations of Fluorescence in situ hybridization (FISH) for identification with various staining techniques targeting functional phenotypes. In this chapter, we describe methods and protocols optimized for the study of extracellular enzymes, surface hydrophobicity and specific surface structures. Although primarily applied to the study of microbes in wastewater treatment (activated sludge and biofilms), the methods may also be used with minor modifications in several other ecosystems.
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© 2010 Humana Press
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Nielsen, J.L., Kragelund, C., Nielsen, P.H. (2010). Ecophysiological Analysis of Microorganisms in Complex Microbial Systems by Combination of Fluorescence In Situ Hybridization with Extracellular Staining Techniques. In: Cummings, S. (eds) Bioremediation. Methods in Molecular Biology, vol 599. Humana Press. https://doi.org/10.1007/978-1-60761-439-5_8
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DOI: https://doi.org/10.1007/978-1-60761-439-5_8
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Online ISBN: 978-1-60761-439-5
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