Skip to main content
Book cover

Advanced Protocols in Oxidative Stress II pp 275–285Cite as

LIBS-Based Detection of Antioxidant Elements: A New Strategy

Part of the Methods in Molecular Biology book series (MIMB,volume 594)

Abstract

The present study deals with the scientific evaluation of antioxidant potential of aqueous extract of Trichosanthes dioica fruits on diabetes-induced oxidative stress of diabetic rats. The most effective dose of mg/kg bw of fruit aqueous extract was given orally to diabetic rats for 30 days. Different oxidative stress parameters were analyzed in various tissues of control and treated diabetic rats. The observed elevated level of lipid peroxidation (LPO) comes down significantly (p < 0.05) and decreased activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) got increased (p < 0.05) significantly of diabetic rats on extract treatment. Laser-Induced Breakdown Spectroscopy (LIBS) has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca2+, Mg2+, and Fe2+, as reflected by their intensities are responsible for antioxidant potential of T. dioica.

Key words

  • Trichosanthes dioica
  • Diabetes
  • LIBS
  • Indian herbs

This is a preview of subscription content, access via your institution.

Protocol
EUR   44.95
Price includes VAT (Finland)
  • DOI: 10.1007/978-1-60761-411-1_19
  • Chapter length: 11 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
EUR   117.69
Price includes VAT (Finland)
  • ISBN: 978-1-60761-411-1
  • Instant EPUB and PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
EUR   159.49
Price includes VAT (Finland)
  • ISBN: 978-1-4939-5703-3
  • Dispatched in 3 to 5 business days
  • Exclusive offer for individuals only
  • Free shipping worldwide
    See shipping information.
  • Tax calculation will be finalised during checkout
Hardcover Book
EUR   109.99
Price includes VAT (Finland)
  • ISBN: 978-1-60761-410-4
  • Dispatched in 3 to 5 business days
  • Exclusive offer for individuals only
  • Free shipping worldwide
    See shipping information.
  • Tax calculation will be finalised during checkout
Learn about institutional subscriptions
Fig. 19.1.
Fig. 19.2.
Fig. 19.3.

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Pasquini C, Cortez J, Silva LMC, Gonzaga FB (2007) Laser induced breakdown spectroscopy. Braz Chem Soc 18:463–512

    CrossRef  CAS  Google Scholar 

  2. Rai AK, Rai VN, Rai DK, Thakur SN, Yueh FU, Singh JP (2007) Laser induced breakdown spectroscopy of solid and molten materials. Elesvier Science, B.V. Chemistry and Chemical Engineering, The Netherlands, pp 255–285

    Google Scholar 

  3. Rai PK, Jaiswal D, Diwaker S, Watal G (2008) Antihyperglycemic profile of Trichosanthes dioica seeds in experimental models. Pharm Biol 46(5):1–6

    CrossRef  Google Scholar 

  4. Rai PK, Rai NK, Rai AK, Watal G (2007) Role of LIBS in elemental analysis of Psidium guajava responsible for glycemic potential. Instrum Sci Tech 35:507–522

    CrossRef  CAS  Google Scholar 

  5. Kar A, Choudhary BK, Bandyopadhyay NG (2003) Comparative evaluation of hypoglycemic activity of some Indian medicinal plants in alloxan diabetic rats. J Ethnopharmacol 84:105–108

    CrossRef  PubMed  Google Scholar 

  6. Beers RF, Sizer IW (1952) A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 195:133–140

    PubMed  CAS  Google Scholar 

  7. Niehaus WG, Samuelsson B (1968) Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation. Eur J Biochem 6:126–130

    CrossRef  PubMed  CAS  Google Scholar 

  8. Ellman GL (1959) A simple, sensitive and reliable method for determining free sulfhydryl content in peptides protein. Arch Biochem Biophys 82:70–77

    CrossRef  PubMed  CAS  Google Scholar 

  9. Warholm M, Guthenberg C, Vonbahr C, Mannervik B (1985) Glutathione transferase from human liver. Meth Enzymol 113:499–504

    CrossRef  PubMed  CAS  Google Scholar 

  10. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin’s phenol reagent. J Biol Chem 193:265–275

    PubMed  CAS  Google Scholar 

  11. Sabsabi M, Cielo P (1995) Quantitative analysis of aluminum alloys by laser-induced breakdown spectroscopy and plasma characterization. Appl Spect 49:499–507

    CrossRef  CAS  Google Scholar 

  12. Giugliano M, Bove M, Grattaro M (2000) Insulin release at the molecular level: metabolic-electrophysiological modeling of the pancreatic beta cells. IEEE Transact Biomed Eng 47:611–623

    CrossRef  CAS  Google Scholar 

  13. Chiang WL, Hsiesh YS, Yang SF, Lu TA, Chu SC (2007) Differential expression of glutathion-S-transferase isoenzymes in various types of anemia in Taiwan. Clin Chem Acta 375:110–114

    CrossRef  CAS  Google Scholar 

  14. Touyz RM, Pu Q, He G, Chen X, Yao G, Neves MF, Viel E (2002) Effects of low dietary magnesium intake on development of hypertension in stroke-prone spontaneously hypertensive rats: role of reactive oxygen species. J Hypertens 20:2221–2232

    CrossRef  PubMed  CAS  Google Scholar 

  15. Bussiere FI, Gueux E, Rock E, Girardeau JP, Tridon A, Mazur A, Rayssiguier Y (2002) Increased phagocytosis and production of reactive oxygen species by neutrophils during magnesium deficiency in rats and inhibition by high magnesium concentration. Brit J Nutr 87:107–113

    CrossRef  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to DRDO, National Medicinal Plants board (NMPB) New Delhi, India, for providing the financial assistance. PKR and DKR are thankful to Indian Council of Medical Research (ICMR) and Council of Scientific and Industrial Research (CSIR) respectively, for the award of SRF to them. The drawing of the experimental setup by Mr. Vivek Kumar Singh, Department of Physics, University of Allahabad is acknowledged.

Author information

Authors and Affiliations

  1. Department of Biochemistry, University of Allahabad, Allahabad, India

    Geeta Watal, Prashant Kumar Rai & Dolly Jaiswal

  2. Medicinal Research Lab, Drug Discovery & Development Division, Alternative Therapeutics Unit, Department of Chemistry, University of Allahabad, Allahabad, India

    Bechan Sharma & Devendra K. Rai

  3. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, India

    Nilesh K. Rai & A. K. Rai

Authors
  1. Geeta Watal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Bechan Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Prashant Kumar Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dolly Jaiswal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Devendra K. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Nilesh K. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. K. Rai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Geeta Watal .

Editor information

Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, USA

    Donald Armstrong

Rights and permissions

Reprints and Permissions

Copyright information

© 2010 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Watal, G. et al. (2010). LIBS-Based Detection of Antioxidant Elements: A New Strategy. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-411-1_19

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-410-4

  • Online ISBN: 978-1-60761-411-1

  • eBook Packages: Springer Protocols