Abstract
The mouse mammary gland is a unique organ since although the mammary gland primordium forms during embryogenesis, the majority of development occurs postnatally upon hormonal stimulation at puberty and full functionality (i.e. lactation) is not achieved until after parturition. Since both the epidermis and mammary glands share the same developmental origin, many mouse models with epidermal phenotypes often also exhibit abnormal mammary gland development. However, since the most widely used methods to analyse mammary glands are laborious and time-consuming, many mouse models exist that have not been analysed for mammary phenotypes. We have developed a simplified method that allows rapid analysis of embryonic mammary anlagen using immunolabelling of whole mounts that should facilitate more comprehensive studies of mouse mammary glands.
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References
Braun KM, Niemann C, Jensen UB, Sundberg JP, Silva-Vargas V, Watt FM. (2003) Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis. Development;130(21):5241–55.
Estrach S, Ambler CA, Lo Celso C, Hozumi K, Watt FM. (2006) Jagged 1 is a beta-catenin target gene required for ectopic hair follicle formation in adult epidermis. Development;133(22):4427–38.
Benitah SA, Frye M, Glogauer M, Watt FM. (2005) Stem cell depletion through epidermal deletion of Rac1. Science;309(5736):933–5.
Mikkola ML, Millar SE. (2006) The mammary bud as a skin appendage: unique and shared aspects of development. J Mammary Gland Biol Neoplasia;11(3–4):187–203.
Howard B, Ashworth A. (2006) Signalling pathways implicated in early mammary gland morphogenesis and breast cancer. PLoS Genet;2(8):e112.
Jolicoeur F, Gaboury LA, Oligny LL. (2003) Basal cells of second trimester fetal breasts: immunohistochemical study of myoepithelial precursors. Pediatr Dev Pathol;6(5):398–413.
Wansbury O, Panchal H, James M, Parry S, Ashworth A, Howard B. (2008) Dynamic expression of Erbb pathway members during early mammary gland morphogenesis. J Invest Dermatol;128(4):1009–21.
Han J, Cao S, Jin H, et al. (2006) Localization of putative stem cells and four cell populations with different differentiation degree in mouse mammary anlagen. Histochem Cell Biol;126(1):35–43.
Nanba D, Nakanishi Y, Hieda Y. (2001) Changes in adhesive properties of epithelial cells during early morphogenesis of the mammary gland. Dev Growth Differ;43(5):535–44.
Cunha GR, Young P, Christov K, et al. (1995) Mammary phenotypic expression induced in epidermal cells by embryonic mammary mesenchyme. Acta Anat (Basel);152(3):195–204.
Sakakura T, Nishizuka Y, Dawe CJ. (1976) Mesenchyme-dependent morphogenesis and epithelium-specific cytodifferentiation in mouse mammary gland. Science;194(4272):1439–41.
Gubbay J, Vivian N, Economou A, Jackson D, Goodfellow P, Lovell-Badge R. (1992) Inverted repeat structure of the Sry locus in mice. Proc Natl Acad Sci USA;89(17):7953–7.
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This work was supported by Breakthrough Breast Cancer.
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Panchal, H., Wansbury, O., Howard, B.A. (2010). Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 585. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-380-0_18
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DOI: https://doi.org/10.1007/978-1-60761-380-0_18
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