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Human Embryonic Stem Cell Protocols pp 425–443Cite as

Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 584))

Abstract

The recent discovery of genomic reprogramming of human somatic cells to an embryonic stem (ES) cell-like pluripotent state provides a unique opportunity for stem cell research. The reprogrammed cells, named as induced pluripotent stem (iPS) cells, possess many of the properties of ES cells and represent one of the most promising sources of patient-specific cells for use in disease model, development of pharmacology and toxicology, screening teratogens, and regenerative medicine. Here we describe the detailed methods for the generation of undifferentiated human iPS (hiPS) cells in feeder layer- and serum-free conditions. This system eliminates direct contact of stem cells with MEFs and reduces use of unknown serum factors that may have undesired activities and enables consistency in large-scale and long-term expansion of undifferentiated hiPS cells. Our findings greatly simplify the method for induction of pluripotency and bring it one step closer to clinical applications. Moreover, the established hiPS cells showed chromosomal stability during long-term culture.

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Acknowledgments

This work was supported by Royan institute.

Author information

Authors and Affiliations

  1. Department of Stem Cells, Cell Science Research Center, Royan Institute, ACECR, Tehran, Iran

    Hossein Baharvand, Mehdi Totonchi, Adeleh Taei, Ali Seifinejad, Nasser Aghdami & Ghasem Hosseini Salekdeh

  2. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran

    Hossein Baharvand

Authors
  1. Hossein Baharvand
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  2. Mehdi Totonchi
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  3. Adeleh Taei
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  4. Ali Seifinejad
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  5. Nasser Aghdami
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  6. Ghasem Hosseini Salekdeh
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Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Ottawa Hosp. Research Institute (OHRI), Smyth Road 501, Ottawa, K1H 8L6, Canada

    Kursad Turksen

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC 2006

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Baharvand, H., Totonchi, M., Taei, A., Seifinejad, A., Aghdami, N., Salekdeh, G.H. (2009). Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions. In: Turksen, K. (eds) Human Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 584. Humana Press. https://doi.org/10.1007/978-1-60761-369-5_23

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  • DOI: https://doi.org/10.1007/978-1-60761-369-5_23

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-368-8

  • Online ISBN: 978-1-60761-369-5

  • eBook Packages: Springer Protocols

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