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Analysis of the NK Cell Immunological Synapse

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Book cover Natural Killer Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 612))

Abstract

Since NK cells specialize in contact-dependent functions including cytotoxicity, interest has focused on the direct study of the interface between the NK cell and the cell with which it is interacting. This interface is also known as the immunological synapse and is characterized by an extraordinary number of dynamic molecular events that have the potential to result in NK cell function. Here we describe microscopy-based methods for evaluating and quantifying the NK cell immunological synapse that can be useful in enabling experimental studies.

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References

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  5. Banerjee, P. P., Pandey, R., Zheng, R., Suhoski, M. M., Monaco-Shawver, L., and Orange, J. S. (2007) Cdc42-interacting protein-4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse. J Exp Med 204, 2305–2320.

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Author information

Authors and Affiliations

  1. Immunology Graduate Group, Division of Immunology, Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Keri B. Sanborn

  2. Cell Biology and Physiology Graduate Group, Division of Immunology, Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine and School of Veterinary Medicine, Philadelphia, PA, USA

    Gregory D. Rak

  3. Cell Biology and Physiology Graduate Group, Division of Immunology, Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    Ashley N. Mentlik

  4. Division of Immunology, Children’s Hospital of Philadelphia, Philadelphia, PA, USA

    Pinaki P. Banerjee

  5. Division of Immunology, The Joseph Stokes Jr. Research Institute, Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine and School of Veterinary Medicine, Philadelphia, PA, USA

    Jordan S. Orange

Authors
  1. Keri B. Sanborn
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  2. Gregory D. Rak
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  3. Ashley N. Mentlik
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  4. Pinaki P. Banerjee
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  5. Jordan S. Orange
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Editor information

Editors and Affiliations

  1. Institute for Cancer Research, Fox Chase Cancer Center, Cottman Avenue 333, Philadelphia, 19111-2497, U.S.A.

    Kerry S. Campbell

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Sanborn, K.B., Rak, G.D., Mentlik, A.N., Banerjee, P.P., Orange, J.S. (2010). Analysis of the NK Cell Immunological Synapse. In: Campbell, K. (eds) Natural Killer Cell Protocols. Methods in Molecular Biology, vol 612. Humana Press. https://doi.org/10.1007/978-1-60761-362-6_9

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  • DOI: https://doi.org/10.1007/978-1-60761-362-6_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-361-9

  • Online ISBN: 978-1-60761-362-6

  • eBook Packages: Springer Protocols

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