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Lipidomics pp 127-159 | Cite as

Lipidomics of the Red Cell in Diagnosis of Human Disorders

  • Peter J. Quinn
  • D. Rainteau
  • C. Wolf
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 579)

Summary

Applications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haemato­logical and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders.

Key words

Lipidomics Diagnosis Erythrocyte Membrane Essential fatty acid deficit Peroxisomal defects Plasmalogen Oxidized phosphatidylethanolamines 

Notes

Acknowledgments

This work was aided by a grant from the Human Frontier Science Programme (RGP0016/2005C).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Peter J. Quinn
    • 1
  • D. Rainteau
    • 2
  • C. Wolf
    • 3
  1. 1.Life Sciences, King’s CollegeLondonUK
  2. 2.UMRS 893-Faculte de Medicine, Pierre et Marie CurieCHU Saint AntoineParisFrance
  3. 3.HDR, Laboratoire de Spectrometrie de Masse, Faculte de Medicine, Pierre et Marie CurieUniversite ParisParisFrance

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