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Lipidomics pp 469-482 | Cite as

The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids

  • Marc Plante
  • Bruce Bailey
  • Ian Acworth
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 579)

Summary

A gradient HPLC-charged aerosol detection method was applied to the measurement of different lipids including: free fatty acids, fatty alcohols, glycerides, steroids, phospholipids, and fat-soluble vitamins. An algal oil sample is used as an example. Charged aerosol detection offers a new approach to the routine analysis of any nonvolatile lipid. It shows low ng (on column) sensitivity, a dynamic range of over four orders of magnitude, good reproducibility, gradient compatibility, and similar analyte response for nonvolatile species, independent of chemical structure. Furthermore, charged aerosol detection uses mobile phases that are fully compatible with LC–MS – enabling both detectors to be used in a “lipidomics platform.”

Key words

Lipids Fatty acids Fatty alcohols Fat-soluble vitamins Biofuels Algal oils Charged aerosol detection Corona HPLC 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Marc Plante
    • 1
  • Bruce Bailey
    • 1
  • Ian Acworth
    • 2
  1. 1.Applications DepartmentESA Biosciences, Inc.ChelmsfordUSA
  2. 2.ESA Biosciences, Inc.ChelmsfordUSA

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