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Primary Dissociated Astrocyte and Neuron Co-culture

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Protocols for Neural Cell Culture

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Abstract

We describe a method to isolate and co-culture dissociated hippocampal neurons and cortical astrocytes from young mice (E17 and newborn, respectively). This protocol is useful to investigate the effects of astrocytes on the developmental biology of neurons. By independently isolating the astrocytes and neurons (with different genetic backgrounds) from different mice, an in vitro environment can be created where one cell type is deficient in a gene or protein of interest. For example, this method is well suited to examine the effect of a genetic mutation in astrocytes on the development of neuronal processes and synapses.

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References

  1. Bartlett WP, Banker GA An electron microscopic study of the development of axons and dendrites by hippocampal neurons in culture II. Synaptic relationships J Neurosci 1984;4:1954–1965.

    CAS  Google Scholar 

  2. Bi G, Poo M Synaptic modifications in cultured hippocampal neurons: dependences on spike timing, synaptic strength and post-synaptic cell type. J Neurosci 1998;18:10464–10472.

    CAS  PubMed  Google Scholar 

  3. Yang Y, Ge W, Chen Y et al. Contribution of astrocytes to hippocampal long term potentiation through release of D-serine. Proc Natl Acad Sci (USA) 2003;11:15194–15195.

    Google Scholar 

  4. Kaech S, Banker G Culturing hippocampal neurons. Nat Protocols 2006;1:2406–2415.

    Article  CAS  Google Scholar 

  5. Yoshida M, Saito H, Katsuki H Neurotrophic effects of conditioned media of astrocytes isolated from different brain regions on hippocampal and cortical neurons. Cell Mol Life Sci 1995;51:133–136.

    Article  CAS  Google Scholar 

  6. Martin DP, Wallace TL, Johnson EM Jr. Cytosine arabinoside kills postmitotic neurons in a fashion resembling trophic factor deprivation: evidence that a deoxycytidine-dependent process may be required for nerve growth factor signal transduction. J Neurosci 1990;10:184–193.

    CAS  PubMed  Google Scholar 

  7. Dessi F, Pollard H, Moreau J et al. Cytosine arabinoside induces apoptosis in cerebellar neurons in culture. J Neurochem 1995;64: 1980–1987.

    Google Scholar 

  8. Chang JY, Brown S Cytosine arabinoside differentially alters survival and neurite outgrowth of neuronal PC12 cells. Biochem Biophys Res Commun. 1996;218:753–758.

    Article  CAS  PubMed  Google Scholar 

  9. Saura J. Microglial cells in astroglial cultures: a cautionary note. J Neuroinflammation 2007;4:26.

    Article  PubMed  Google Scholar 

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Acknowledgments

We thank Stephanie Kaech-Petrie for her tireless advice that was instrumental in the development of this protocol.This research is supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and the Fragile X Research Foundation of Canada.

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Jacobs, S., Doering, L.C. (2009). Primary Dissociated Astrocyte and Neuron Co-culture. In: Doering, L. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60761-292-6_16

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  • DOI: https://doi.org/10.1007/978-1-60761-292-6_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-291-9

  • Online ISBN: 978-1-60761-292-6

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