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Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine

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Reverse Chemical Genetics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 577))

Summary

Two-dimensional difference gel electrophoresis (2D-DIGE) is an advanced variation of two-dimensional polyacrylamide gel electrophoresis (2D-PAGE); protein samples are labeled with different fluorescent dyes, mixed and separated by 2D-PAGE. 2D-DIGE solves major inherent drawbacks of 2D-PAGE, demonstrating great utility in biomarker studies. Biomarker development requires quantitative, reproducible, highly sensitive and high-throughput experimental platforms, and 2D-DIGE meets these criteria. Here we demonstrate the advantages of 2D-DIGE and discuss the possibilities 2D-DIGE offers for further, more comprehensive proteome studies.

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Acknowledgments

This work was supported by a grant from the Ministry of Health, Labor, and Welfare and by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation of Japan. We appreciate Dr. Hisao Asamura (National Cancer Center Hospital) for an excellent photograph of surgical operation.

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Authors and Affiliations

  1. National Cancer Center Research Institute, Proteome Bioinformatics Project, Tokyo, Japan

    Tadashi Kondo & Setsuo Hirohashi

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  1. Tadashi Kondo
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  2. Setsuo Hirohashi
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Editors and Affiliations

  1. Iwai Orthopaedic Medical Hospital, Minamikoiwa 8-17-5, Tokyo, 133-0055, Japan

    Hisashi Koga

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Kondo, T., Hirohashi, S. (2009). Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine. In: Koga, H. (eds) Reverse Chemical Genetics. Methods in Molecular Biology™, vol 577. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-232-2_11

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  • DOI: https://doi.org/10.1007/978-1-60761-232-2_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-231-5

  • Online ISBN: 978-1-60761-232-2

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