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Proteomics pp 143–153Cite as

Multidimensional Protein Identification Technology

Part of the Methods in Molecular Biology™ book series (MIMB,volume 564)

Summary

Over the past years, large-scale analysis of proteomes gained increased interest to obtain a fast but nevertheless comprehensive overview about cellular protein content. While a complete proteome cannot be covered using current technologies because of its enormous diversity, subfractionation to reduce the complexity has become mandatory. While 2D-PAGE is well established as a high-resolution protein separation technique, it suffers from drawbacks, which can be overcome by using peptide separation methods based on multidimensional liquid chromatography. One of these technologies is multidimensional protein identification technology (MudPIT). It consists of two orthogonal separation systems – strong cation exchange (SCX) and reversed phase (RP) – coupled online in an automated fashion to mass spectrometric detection. This method offers the possibility to analyze high-complex peptide mixtures in a single experiment.

Key words

  • MudPIT
  • Liquid chromatography
  • Peptide separation
  • Mass spectrometry

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Correspondence to Dirk Wolters .

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Lohrig, K., Wolters, D. (2009). Multidimensional Protein Identification Technology. In: Reinders, J., Sickmann, A. (eds) Proteomics. Methods in Molecular Biology™, vol 564. Humana Press. https://doi.org/10.1007/978-1-60761-157-8_8

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  • DOI: https://doi.org/10.1007/978-1-60761-157-8_8

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  • Print ISBN: 978-1-60761-156-1

  • Online ISBN: 978-1-60761-157-8

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