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Protocols for Adult Stem Cells pp 117–137Cite as

Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix

Part of the Methods in Molecular Biology™ book series (MIMB,volume 621)

Abstract

Mesenchymal stem cells (MSCs) have huge potential to be used in cell therapies because of their pluripotency and immunoregulatory attributes. To harness and maximize the therapeutic potential of MSCs, a thorough understanding of their differentiation pathways and their responses to the microenvironment is needed. The matrix that the cells reside on is an important microenvironmental cue, not only for its chemical components but also for its physical properties. The rigidity and topography of the matrix are two physical factors that are crucial in directing cell behavior. In this chapter, we describe the isolation, culture, and characterization of MSCs. Then we illustrate the procedure of creating different matrix rigidities using acrylamide gels for MSC differentiation study. Finally, we describe how to create MSC-seeded vascular grafts using scaffolds with nano topographical features.

Key words

  • Mesenchymal stem cell
  • Bone marrow
  • Acrylamide gel
  • Rigidity
  • Stiffness
  • Nanofiber
  • Vascular graft
  • Topography
  • Young’s modulus

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Authors and Affiliations

  1. Department of Bioengineering, University of California, Berkeley, CA, USA

    Jennifer S. Park, Craig Hashi & Song Li

  2. Joint Graduate Group in Bioengineering, University of California, San Francisco and Berkeley, CA, USA

    Jennifer S. Park & Craig Hashi

Authors
  1. Jennifer S. Park
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  2. Craig Hashi
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  3. Song Li
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Corresponding author

Correspondence to Song Li .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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Park, J.S., Hashi, C., Li, S. (2010). Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_8

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

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