Abstract
The targeted destruction of key regulators helps to drive the cell cycle. Here we describe a quantitative assay to measure destruction of different regulators in mitotic cells. This assay uses GFP-tagged substrates and time-lapse fluorescence microscopy of single cells to pinpoint the timing of destruction of different substrates at different stages in mitosis.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Lindon, C., Fiore, B.D. (2009). Measuring Proteolysis in Mitosis. In: McAinsh, A. (eds) Mitosis. Methods in Molecular Biology, vol 545. Humana Press. https://doi.org/10.1007/978-1-60327-993-2_16
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DOI: https://doi.org/10.1007/978-1-60327-993-2_16
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