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Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands

  • Michael J. McGuire
  • Shunzi Li
  • Kathlynn C. Brown
Part of the Methods in Molecular Biology™ book series (MIMB, volume 504)

Summary

One limitation in the development of biosensors for the early detection of disease is the availability of high specificity and affinity ligands for biomarkers that are indicative of a pathogenic process. Within the past 10 years, biopanning of phage displayed peptide libraries on intact cells has proven to be a successful route to the identification of cell-specific ligands. The peptides selected from these combinatorial libraries are often able to distinguish between diseased cells and their normal counterparts as well as cells in different activation states. These ligands are small and chemical methodologies are available for regiospecific derivatization. As such, they can be incorporated into a variety of different diagnostic and therapeutic platforms. Here we describe the methods utilized in the selection of peptides from phage displayed libraries by biopanning. In addition, we provide methods for the synthesis of the selected peptides as both monomers and tetramers. Downstream uses for the peptides are illustrated.

Key words

Phage display Peptides Cell-targeting Biopanning Combinatorial library Diagnostics Therapeutics Quantum dots 

Notes

Acknowledgments

This work was supported by the National Cancer Institute of the NIH (1RO1CA106646 and R211R21CA114157-01).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Michael J. McGuire
    • 1
  • Shunzi Li
    • 1
  • Kathlynn C. Brown
    • 1
  1. 1.Division of Translational Research, Department of Internal Medicine and Simmons Comprehensive Cancer CenterUniversity of Texas Southwestern Medical CenterDallasUSA

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