Rapid Prototyping of Lateral Flow Assays

  • Alexander Volkov
  • Michael Mauk
  • Paul Corstjens
  • R. Sam Niedbala
Part of the Methods in Molecular Biology™ book series (MIMB, volume 504)


Principles and characteristics of lateral flow strip assays are reviewed. Recent technology developments permit the use of inexpensive electronic readers for interrogating lateral flow strip test results, thus avoiding the inevitable variation and subjectivity of visual inspection to assess the capture of reporter-labeled analyte on test lines of the strip. Protocols for developing lateral flow assays are described, including two specific case studies for assaying cotinine (a small-molecule metabolite of nicotine) in a competitive format, and assaying HIV antibodies in a sandwich-type assay format.

Key words

Lateral flow assay Rapid assay development Nitrocellulose strips Immunochromatography 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Alexander Volkov
    • 1
  • Michael Mauk
    • 2
  • Paul Corstjens
    • 3
  • R. Sam Niedbala
    • 1
  1. 1.Department of ChemistryLehigh UniversityBethlehemUSA
  2. 2.School of Engineering and Applied ScienceUniversity of PennsylvaniaPhiladelphiaUSA
  3. 3.Leiden University Medical CenterLeidenThe Netherlands

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