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Biosensors and Biodetection pp 169–183Cite as

Immunochromatographic Lateral Flow Strip Tests

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 504))

Summary

The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus; (b) Trichinella specific antibodies, and (c) Clenbuterol residues in urine samples.

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Acknowledgments

This work was supported by grants from the National Basic Research Program of China (Grant No. 2005CB523203) and the Chinese High Technology Research and Development Project (Grant No. 2001AA249031). The authors thank Dr. Norman A. Gregson at King's College, University of London for his critical review of this manuscript.

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Authors and Affiliations

  1. The Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China

    Gaiping Zhang, Junqing Guo & Xuannian Wang

Authors
  1. Gaiping Zhang
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  2. Junqing Guo
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  3. Xuannian Wang
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Editor information

Editors and Affiliations

  1. FDA Center for Devices and Radiological Health, Silver Spring, MD, USA

    Avraham Rasooly

  2. National Cancer Institute, Bethesda, MD, USA

    Avraham Rasooly

  3. Fischell Department of Bioengineering, University of Maryland, College Park, MD, USA

    Keith E. Herold

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© 2009 Springer Science+Business Media, LLC

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Zhang, G., Guo, J., Wang, X. (2009). Immunochromatographic Lateral Flow Strip Tests. In: Rasooly, A., Herold, K.E. (eds) Biosensors and Biodetection. Methods in Molecular Biology™, vol 504. Humana Press. https://doi.org/10.1007/978-1-60327-569-9_12

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  • DOI: https://doi.org/10.1007/978-1-60327-569-9_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-568-2

  • Online ISBN: 978-1-60327-569-9

  • eBook Packages: Springer Protocols

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