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Immunochromatographic Lateral Flow Strip Tests

Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 504)

Summary

The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus; (b) Trichinella specific antibodies, and (c) Clenbuterol residues in urine samples.

Key words

Immunochromatography Lateral flow strip test Infectious bursal disease virus Trichinella Clenbuterol 
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Notes

Acknowledgments

This work was supported by grants from the National Basic Research Program of China (Grant No. 2005CB523203) and the Chinese High Technology Research and Development Project (Grant No. 2001AA249031). The authors thank Dr. Norman A. Gregson at King's College, University of London for his critical review of this manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.The Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural SciencesZhengzhouPeople's Republic of China

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