A Set of Piezoelectric Biosensors Using Cholinesterases

  • Carsten Teller
  • Jan Halámek
  • Alexander Makower
  • Frieder W. Scheller
Part of the Methods in Molecular Biology™ book series (MIMB, volume 504)


Piezoelectric sensors have become a versatile tool in biosensorics to study protein—protein and protein—small molecule interactions. Here we present theoretical background on piezoelectric sensors and instructions, how to modify their surface with various recognition elements for cholinesterases. These recognition elements comprise an organophosphate (paraoxon), a cocaine derivative (BZE-DADOO), and a tricyclic, aromatic compound (propidium). Additionally, a guide to the kinetic evaluation of the obtained binding curves is given in this chapter.

Key words

Piezoelectric biosensors QCM Cholinesterase Pesticides Drugs Antibody Kinetic evaluation. 



The authors thank the European Union (Marie-Curie-fellowship HPMD-CT-2001-00062) and the “International Max Planck Research School on Biomimetic Systems” (PhD scholarship) for financial support.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Carsten Teller
    • 1
  • Jan Halámek
    • 2
  • Alexander Makower
    • 1
  • Frieder W. Scheller
    • 1
  1. 1.Department of Analytical BiochemistryUniversity of PotsdamPotsdamGermany
  2. 2.Department of Analytical BiochemistryUniversity of Potsdam, Potsdam, Germany Biophysical Engineering Group, University of TwenteEnschedeThe Netherlands

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