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Reflectometric Interference Spectroscopy

  • Guenther Proll
  • Goran Markovic
  • Lutz Steinle
  • Guenter Gauglitz
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

Summary

Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance (SPR). The advantages of direct optical detection depend on a sophisticated surface chemistry resulting in negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.

Key words

Label-free optical biosensor Reflectometric interference spectroscopy (RIfS) 

References

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Guenther Proll
    • 1
  • Goran Markovic
    • 1
  • Lutz Steinle
    • 1
  • Guenter Gauglitz
    • 1
  1. 1.Institute of Physical and Theoretical ChemistryUniversity of TuebingenTuebingenGermany

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