Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection

  • Jong Seol Yuk
  • Kwon-Soo Ha
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)


Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x–y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases.

Key words

Spectral SPR biosensor Line scanning mode Protein array Mumps virus Serodiagnosis Resonance wavelength PDDA 



This work was supported in part by a grant of the Korea Health 21 R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A030003).


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jong Seol Yuk
    • 1
  • Kwon-Soo Ha
    • 1
  1. 1.Department of Molecular and Cellular Biochemistry and Nanobio Sensor Research CenterKangwon National University College of MedicineChuncheonKorea

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