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Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection

  • Jong Seol Yuk
  • Kwon-Soo Ha
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

Summary

Spectral SPR biosensor is a useful system for a rapid analysis of protein arrays, as the biosensor with a fiber optic spectrometer can be easily aligned with the reflected light from protein arrays. The spectral SPR biosensor was constructed by Kretschmann geometry, based on the wavelength interrogation with various modules such as protein arrays, optical unit, programs for data acquisition and processing, and a motorized x–y stage for scanning. Protein arrays consist of glass/gold film/linker layer/protein/air. The surface of gold arrays was modified with poly(diallyldimethylammonium chloride) and 11-mercap-toundecanoic acid to immobilize mumps virus. The virus arrays were used to analyze anti-mumps virus in a buffer or human serum by the line-scanning mode of the spectral SPR biosensor. The array-based spectral SPR biosensor has a strong potential for a high-throughput serodiagnosis of infectious diseases.

Key words

Spectral SPR biosensor Line scanning mode Protein array Mumps virus Serodiagnosis Resonance wavelength PDDA 

Notes

Acknowledgments

This work was supported in part by a grant of the Korea Health 21 R&D Project, Ministry of Health, Welfare & Family Affairs, Republic of Korea (A030003).

References

  1. 1.
    Liedberg, B., Nylander, C., and Lundström, I. (1983) Surface plasmon resonance for gas detection and biosensing. Sens. Actuators B 4, 299–304CrossRefGoogle Scholar
  2. 2.
    Yuk, J. S. and Ha, K. -S. (2005) Proteomic applications of SPR biosensors: analysis of protein arrays. Exp. Mol. Med. 37, 1–10PubMedGoogle Scholar
  3. 3.
    Ha, K. -S. and Yuk, J. S. (2006) Wavelength interrogation-based surface plasmon reso nance biosensors, in Encyclopedia of Sensors (Grimes, C. A., Dickey, E. C., and Pishko, M. V., eds), American Scientific, Stevenson Ranch, CA, pp. 433–442Google Scholar
  4. 4.
    Homola, J., Yee, S. S., and Gauglitz, G. (1999) Surface plasmon resonance sensors: review. Sens. Acuators B54, 3–15CrossRefGoogle Scholar
  5. 5.
    Yuk, J. S., Jung, S. -H., Jung, J. -W., Hong, D. -G., Han, J. -A., Kim, Y. -M., and Ha, K. -S. (2004) Analysis of protein interactions by a wavelength interrogation-based surface plasmon resonance biosensor. Proteomics 4, 3468–3476CrossRefPubMedGoogle Scholar
  6. 6.
    Röthenhausler, B., and Knoll, W. (1998) Surfac-plasmon microscopy. Nature 332, 615–617CrossRefGoogle Scholar
  7. 7.
    Yuk, J. S., Kim, H. -S., Jung, J. -W., Jung, S. -H., Han, J. A., Kim, Y. -M., and Ha, K. -S. (2006) Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging. Biosens. Bioelectron. 21, 1521–1528CrossRefPubMedGoogle Scholar
  8. 8.
    Yuk, J. S., Yi, S. -J., Lee, H. J., Lee, H. G., Kim, Y. -M., and Ha, K. -S. (2003) Characterization of surface plasmon resonance wavelength by changes of protein concentration on protein chips. Sens. Actuators B94, 161–164CrossRefGoogle Scholar
  9. 9.
    Jung, J. -W., Jung, S. -H., Kim, H. -S., Yuk, J. S., Park, J. -B., Kim, Y. -M., Han, J. -A., Kim, P. -H., and Ha, K. -S. (2006) High-throughput analysis of GST-fusion protein expression and activity-dependent protein interactions on GST-fusion protein arrays by using a spectral surface plasmon resonance biosensor. Proteomics 6, 1110–1120CrossRefPubMedGoogle Scholar
  10. 10.
    Kim, H. -S., Jung, S. -H., Kim, S. -H., Suh, I. B., Kim, W. J., Jung, J. -W., Yuk, J. S., Kim, Y. -M., and Ha, K. -S. (2006) High-throughput analysis of mumps virus and the virus specific monoclonal antibody on the arrays of a cationic polyelectrolyte with a spectral SPR biosensor. Proteomics 6, 6426–6432CrossRefPubMedGoogle Scholar
  11. 11.
    Kim, S. H., Kee, S. H., Ahn, J. -E., Song, J. -W., Song, K. -J. (2003) Production and characterization of monoclonal antibodies to mumps virus isolated in Korea. J. Bacteriol. Virol. 33, 203–208Google Scholar
  12. 12.
    Yuk, J. S. and Ha, K. -S. (2004) Analysis of immunoreactions on protein arrays by using wavelength interrogation-based surface plasmon resonance sensors. J. Korean Phys. Soc. 45, 1104–1108Google Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jong Seol Yuk
    • 1
  • Kwon-Soo Ha
    • 1
  1. 1.Department of Molecular and Cellular Biochemistry and Nanobio Sensor Research CenterKangwon National University College of MedicineChuncheonKorea

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