Advertisement

Multiplex Integrating Waveguide Sensor: Signalyte™-II

  • Shuhong Li
  • Yunqi Zhang
  • Platte AmstutzIII
  • Cha-Mei Tang
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

A platform to detect multiplex fluorescent labels was developed based on liquid phase implementation of the Integrating Waveguide Sensor detection principles. The liquid sample is held in a capillary cuvette with a lens at one end. The excitation light incident on the cuvette at 90° angle. The emitted fluores cence is efficiently gathered and propagated to the end of the waveguide cuvette, exiting via the lens to the detector. The capillary cuvette acts as a waveguide to efficiently gather the emission signal, providing high detection sensitivity for small sample sizes.

Excitation sources ranging from 470 to 635 nm are four high-powered LEDs, allowing for multiplex fluorescence assays and a spectrometer is used to collect the signal from 390 to 790 nm. The cuvette can hold 1–35 μL samples. This technology can be used for a wide variety of assays and detection needs, such as FRET, end point PCR reading, immunoassays, chemiluminescence detection, multiplex quantum dots assays, polarization assays, etc.

Keywords

Biosensor Spectrofluorometer Fluorometer Integrating waveguide sensor 

References

  1. 1.
    Feldstein, M. J., MacCraith, B. D., Ligler, F. S., US Patent No. 6,137,117 issued on 10/24/2000Google Scholar
  2. 2.
    Ligler, F. S., Breimer, M., Golden, J. P. , Nivens, D. A., Dodson, J. P., Green, T. M., Haders, D. P., Sadik, O. A., Integrating waveguide sensor. Anal. Chem. 74, 713–719, 2002CrossRefPubMedGoogle Scholar
  3. 3.
    Li, S., Amstutz, P., Tang, C. M., Hang, J., Zhu, P., Zhang, Y., Shelton, D. R., and Karns, J. S., Integrating Waveguide Sensor, Chapter 22 in this bookGoogle Scholar
  4. 4.
    Tang, C.-M. and Amstutz, P. , III, Sensitive Emission Light Gathering and Detection System, US Patent Application, filed on October 3, 2006Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shuhong Li
    • 1
  • Yunqi Zhang
    • 1
  • Platte AmstutzIII
    • 1
  • Cha-Mei Tang
    • 1
  1. 1.Creatv MicroTech, Inc.PotomacUSA

Personalised recommendations