Integrating Waveguide Biosensor
The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90° angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells of Salmonella.
KeywordsBiosensor Integrating waveguide sensor Integrating waveguide biosensor Fluorescence detection Salmonella
We would like to thank Dr. Francis Ligler (Naval Research Laboratory, Washington DC) for helpful advice and comments. This work was supported by NIH Small Business Innovation Research grants R43 CA094430 and R43 AI052684 and a grant from the Maryland Technology Development Corporation (TEDCO).
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