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Integrating Waveguide Biosensor

  • Shuhong Li
  • Platte AmstutzIII
  • Cha-Mei Tang
  • Jun Hang
  • Peixuan Zhu
  • Yunqi Zhang
  • Daniel R. Shelton
  • Jeffrey S. Karns
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed using Salmonella as model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90° angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells of Salmonella.

Keywords

Biosensor Integrating waveguide sensor Integrating waveguide biosensor Fluorescence detection Salmonella 

Notes

Acknowledgments

We would like to thank Dr. Francis Ligler (Naval Research Laboratory, Washington DC) for helpful advice and comments. This work was supported by NIH Small Business Innovation Research grants R43 CA094430 and R43 AI052684 and a grant from the Maryland Technology Development Corporation (TEDCO).

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shuhong Li
    • 1
  • Platte AmstutzIII
    • 1
  • Cha-Mei Tang
    • 1
  • Jun Hang
    • 1
  • Peixuan Zhu
    • 1
  • Yunqi Zhang
    • 1
  • Daniel R. Shelton
    • 2
  • Jeffrey S. Karns
    • 2
  1. 1.Creatv MicroTech, Inc.PotomacUSA
  2. 2.U.S. Department of Agriculture-Agricultural Research ServiceEnvironmental Microbial Safety LaboratoryBeltsvilleUSA

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