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Miniaturized and Integrated Fluorescence Detectors for Microfluidic Capillary Electrophoresis Devices

  • Toshihiro Kamei
Part of the Methods in Molecular Biology™ book series (MIMB, volume 503)

Summary

Microfluidic devices are revolutionary in their ability to use very small quantities of liquid samples and to perform biochemical analyses with unprecedented speed. Toward the goal of a lab-on-a-chip that integrates a series of analysis steps and analytical components into a single microfluidic device, one of the most critical aspects of size reduction is miniaturizing and integrating the fluorescence detection system. We present here details of a new integrated fluorescence detection system. A microfluidic biochemical analysis device is mounted on a compact detection platform that comprises a fluorescence-collecting microlens and micromachined fluorescence detector in which a multilayer optical interference filter is monolithically integrated and patterned on a hydrogenated amorphous silicon (a-Si:H) photodiode. A central aperture in the micromachined a-Si:H fluorescence detector allows semiconductor laser light to pass up through the detector and to irradiate a microchannel of the microfluidic analysis device. Such an optical configuration enables a detachable, reusable, compact module to be constructed for the excitation source and detector. The micromachined a-Si:H fluorescence detector exhibits high sensitivity for practical fluorescent labeling dye, making it ideal for application to portable point-of-care microfluidic biochemical analysis devices.

Key words

Microfluidic Lab-on-a-chip Hydrogenated amorphous silicon Photodiode Fluorescence Electrophoresis 

Notes

Acknowledgments

This study was carried out partly in collaboration with Prof. Richard Mathies' group at University of California in Berkeley. I would like to particularly thank Dr. James Scherrer for his contribution to construct the optical setup and Dr. Brian Paegel for his consultation concerning microfluidic electrophoresis. This work was supported in part by New Energy and Industrial Technology Development Organization (NEDO) of Japan.

References

  1. 1.
    Harrison, D.J., Fluri, K., Seiler, K., Fan, Z.H., Effenhauser, C.S., and Manz, A.(1993) Micromachining a miniaturized capillary electrophoresis-based chemical-analysis system on a chip.Science 261, 895–97CrossRefPubMedGoogle Scholar
  2. 2.
    Unger, M.A., Chou, H.P., Thorsen, T., Scherer, A., and Quake, S.R.(2000) Monolithic micro-fabricated valves and pumps by multilayer soft lithography.Science 288, 113–16CrossRefPubMedGoogle Scholar
  3. 3.
    Fu, A.Y., Chou, H.P., Spence, C., Arnold, F.H., and Quake, S.R.(2002) An integrated microfabricated cell sorter.Analytical Chemistry 74, 2451–57CrossRefPubMedGoogle Scholar
  4. 4.
    Hansen, C.L., Skordalakes, E., Berger, J.M., and Quake, S.R.(2002) A robust and scalable microfluidic metering method that allows protein crystal growth by free interface diffusion.Proceedings of the National Academy of Sciences of the United States of America 99, 16531–36CrossRefPubMedGoogle Scholar
  5. 5.
    Kamei, T., Paegel, B.M., Scherer, J.R., Skelley, A.M., Street, R.A., and Mathies, R.A.(2003) Integrated hydrogenated amorphous Si photodiode detector for microfluidic bio-analytical devices.Analytical Chemistry 75, 5300–05CrossRefPubMedGoogle Scholar
  6. 6.
    Kamei, T., and Wada, T.(2006) Contact-lens type of micromachined hydrogenated amorphous Si fluorescence detector coupled with microfluidic electrophoresis devices.Applied Physics Letters 89, 114101CrossRefGoogle Scholar
  7. 7.
    Kamei, T., Toriello, N.M., Lagally, E.T., Blazej, R.G., Scherer, J.R., Street, R.A., and Mathies, R.A.(2005) Microfluidic genetic analysis with an integrated a-Si: H detector.Biomedical Microdevices 7, 147–52CrossRefPubMedGoogle Scholar
  8. 8.
    Lagally, E.T., Lee, S.H., and Soh, H.T.(2005) Integrated microsystem for dielec-trophoretic cell concentration and genetic detection.Lab on a Chip 5, 1053–58CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Toshihiro Kamei
    • 1
  1. 1.National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan

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